Table 4 Number of mutations and mutation frequency of recoded HIV-1 variants after 15 passages in MT-4 cells
Virus | Synonymous mutations (reversions)a | Nonsynonymous mutations | Mutations/Nucleotides | Mutation frequency | |
|---|---|---|---|---|---|
Replicate 1 | HIV-Pwtp15 | 3 | 9 | 12/6831 | 1.76 × 10-3 |
HIV-Pmaxp15 | 2(2) | 7 | 9/6237 | 1.44 × 10-3 | |
HIV-PminAp15 | 23(21) | 9 | 32/7128 | 4.49 × 10-3 | |
Replicate 2 | HIV-Pwtp15 | 2 | 10 | 12/6237 | 1.92 × 10-3 |
HIV-Pmaxp15 | 0 | 5 | 5/5049 | 0.99 × 10-3 | |
HIV-PminAp15 | 6(1) | 12 | 18/5940 | 3.03 × 10-3 | |
Replicate 1 | HIV-GwtAp15 | 9 | 8 | 17/7875 | 2.16 × 10-3 |
HIV-GminAp15 | 60(25) | 59 | 119/6375 | 1.87 × 10-2 | |
HIV-GwtBp15 | 7 | 11 | 18/8421 | 2.14 × 10-3 | |
HIV-GminBp15 | 186(172) | 5 | 191/7600 | 2.51 × 10-2 | |
HIV-GwtCp15 | 24 | 6 | 30/8400 | 3.57 × 10-3 | |
HIV-GminCp15 | 71(41) | 26 | 97/8800 | 1.10 × 10-2 | |
HIV-GwtDp15 | 6 | 24 | 30/8190 | 3.66 × 10-3 | |
HIV-GminDp15 | 13(1) | 15 | 28/4680 | 5.98 × 10-3 | |
Replicate 2 | HIV-GwtAp15 | 9 | 17 | 26/6375 | 4.08 × 10-3 |
HIV-GminAp15 | 82(39) | 78 | 160/8250 | 1.94 × 10-2 | |
HIV-GwtBp15 | 8 | 16 | 24/6000 | 4.00 × 10-3 | |
HIV-GminBp15 | 250(233) | 8 | 250/9200 | 2.72 × 10-2 | |
HIV-GwtCp15 | 13 | 8 | 21/3600 | 5.83 × 10-3 | |
HIV-GminCp15 | 45(20) | 19 | 64/4000 | 1.60 × 10-2 | |
HIV-GwtDp15 | 19 | 15 | 32/5460 | 5.86 × 10-3 | |
HIV-GminDp15 | 18(1) | 24 | 42/4680 | 8.55 × 10-3 |