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Table 2 Real-time quantitative RT-PCR analysis of HIV-1 gag (p24) and GFP gene RNAs after one cycle of viral replication

From: Changes in codon-pair bias of human immunodeficiency virus type 1 have profound effects on virus replication in cell culture

 

HIV-Pwt

HIV-Pmax

HIV-PminA

HIV-PminB

HIV-PminC

HIV-PminBC

GFP Cta

17.01 ± 0.05

17.22 ± 0.07

17.97 ± 0.04

17.15 ± 0.07

17.10 ± 0.14

17.14 ± 0.02

gag Ct

17.89 ± 0.39

17.40 ± 0.37

18.02 ± 0.36

17.36 ± 0.66

17.53 ± 0.14

17.86 ± 0.19

GAPDH Ct

16.46 ± 0.04

16.25 ± 0.23

16.52 ± 0.30

16.65 ± 0.62

16.49 ± 0.31

16.22 ± 0.04

2^(−ΔΔCt)b gag/GADPH

1.00

1.22

0.95

1.66

1.32

0.91

2^(−ΔΔCt) gag/GFP

1.00

0.94

0.96

0.94

0.91

0.97

  1. aCt, threshold cycle.
  2. bLivak formula for relative gene expression analysis.
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Retrovirology

ISSN: 1742-4690

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