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Figure 5 | Retrovirology

Figure 5

From: Changes in codon-pair bias of human immunodeficiency virus type 1 have profound effects on virus replication in cell culture

Figure 5

Replication kinetic assay of HIV-1 recoded variants after 15 serial passages in MT-4 cells. (A) Protease variants, (B) gag variants. Passages were started with a MOI, 0.0002. Two replicates were performed for each variant. Production of the HIV-1 antigen p24 in culture supernatants was determined on days 0–5. For each virus, the slope of the plot provides an estimate of the viral replication capacity. The slope of the p24 antigen production of each virus after infection of MT-4 cells is shown by the bars. Comparisons between wild-type (HXB2) and mutant recoded viruses are shown. The significance of the difference between slopes was calculated with GraphPrism v. 4 software (unpaired t-test). Values represent the mean ± SD from at least three independent experiments.

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