Figure 7
From: Heterogeneous susceptibility of circulating SIV isolate capsids to HIV-interacting factors
Optimal infection of human cells mediated by both HIV-1 and HIV-2 capsids is dependent on Nup153 while capsids of SIVmac and circulating SIV capsids are mildly or not dependent on Nup153. (A) Specific down modulation of Nup153 in HEK-293T cells (siRNANup153) as compared to control siLuc-treated cells. Cells were analyzed as described in the Figure 6 legend. (B) Infection of Nup153-depleted HEK-293T cells as compared to infection of control siLuc-treated cells were determined as described above using MLV-B, wt and chimeric HIV-1 constructs, HIV-2ROD, SIVmac, and circulating SIV CA constructs. Results were obtained from at least three independent experiments with each CA. Unpaired two-tailed Student’s t-test was used to assess significance. SEM and P-values (* P < 0.05, *** P < 0.001) are indicated.