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Figure 4 | Retrovirology

Figure 4

From: Heterogeneous susceptibility of circulating SIV isolate capsids to HIV-interacting factors

Figure 4

Natural primate TRIMCypA fusion proteins show heterogeneous patterns of restriction against circulating SIV CA. (A) Highly conserved sequences between human CypA and the carboxy terminal CypA domains of primate TRIM5CypA fusion proteins; also shown is the related, but more divergent, carboxy terminal Cyp-like domain of the Nup158/RanBP2 nucleoporin (see text); (−) identical residues. (B) Restriction pattern of a CHO cell line stably expressing owl-TRIMCypA fusion protein against different lentiviral capsids as compared to infection of control CHO cells. (C) assessment of CypA dependence of the owl-TRIMCypA restriction against prototypic wt and mutant HIV-1 capsid constructs and against two circulating SIV capsids, assayed in the absence (−) or the presence (+) of cyclosporine A (CsA), a CypA inhibitor. (D) Infection titers of chimeric CA viruses on CHO cells stably expressing either TRIMCypA from Maccaca fascicularis (mafa-TRIMCypA) or (E) TRIMCypA from Maccaca mulatta (mamu-TRIMCypA) were compared to titers on control CHO cells as described above in (B) with the additional use of a chimeric SIVmac GagPol construct containing MACA of HIV2ROD as control. Infectivity was calculated as indicated in Figure 2. All results were compiled from at least three independent experiments for each combination. Unpaired two-tailed Student’s t-test was used to assess significance. SEM and P-values < 0.001 (asterisks) are indicated.

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