Figure 11
From: Identification of the feline foamy virus Bet domain essential for APOBEC3 counteraction
Colocalization of feA3Z2a with nonfunctional Bet proteins. HeLa cells were transfected with a plasmid encoding HA-tagged feA3Z2a-HA (A) and/or Bet expression plasmids (B – F), as indicated on the right. feA3Z2a-HA was detected with HA tag-specific antibody (green), Bet proteins were detected by an FFV Bet-specific serum (red) and nuclei were stained with Hoechst 33342 (blue). The merge of the feA3Z2a and Bet staining is shown in the right-hand column. Nonfunctional Bet proteins are localized in the cytoplasm (B, D, F) while feA3Z2a is localized both in the cytoplasm and the nucleus (A). In cells coexpressing feA3Z2a and a nonfunctional Bet mutant, there is no relocalization of proteins (C, E, G). Inserted bars represent 5 μm.