Figure 10

Colocalization of feA3Z2a with functional Bet proteins. HeLa cells were transfected with a plasmid encoding HA-tagged feA3Z2a-HA (A) and/or Bet expression plasmids (wt Bet or FFV-PFV Bet CH1, B – H), as indicated on the right. feA3Z2a-HA was detected with HA tag-specific antibody (green), FFV Bet was detected by an anti-V5 tag antibody (red) and nuclei were stained with Hoechst 33342 (blue). The merge of feA3Z2a and Bet staining is shown in the right-hand column. Wt Bet and FFV-PFV CH1 are localized in the cytoplasm (B, E) while feA3Z2a is localized both in the cytoplasm and the nucleus (A). In some cells coexpressing feA3Z2a and functional Bet proteins, wt Bet and CH1 relocalize to the nucleus (C, F, G). In other cells, feA3Z2a is recruited to the cytoplasm (D, H). Inserted bars represent 5 μm.