Figure 7From: Acetylation at lysine 346 controls the transforming activity of the HTLV-1 Tax oncoprotein in the Rat-1 fibroblast modelAcetylation controls Tax-mediated release of free E2F. CHO cells were transfected with 250Â ng of p3xE2F-WT-luc reporter construct and 100Â ng of pRL-TK-luc, 50Â ng of vectors expressing cyclin D3 and CDK4 alone or in combination with vectors expressing p21 (100Â ng), and either WT Tax or the K346R or K346Q mutants (600Â ng). The results are presented as fold increase of luciferase activities. The values are averages of three independent experiments, with standard deviations indicated.Back to article page