Figure 2

Colocalization of Tax with p300 and HDAC7 in the Tax nuclear bodies modulates Tax acetylation status. HeLa cells were transfected with vectors expressing WT Tax or the K₃₄₆R mutant alone (A) or with a vector expressing either p300-HA (B) or HDAC7-Flag (C). Cells were analyzed by triple immunofluorescence staining and confocal microscopy with the anti-Tax IgG2a mAb, the anti-AcK₃₄₆Tax rabbit polyclonal antibody and either the anti-p300 IgG1 mAb (A), the anti-HA IgG1 mAb for the detection of overexpressed p300-HA (B) or the anti-Flag IgG1 mAb for the detection of HDAC7-Flag (C). The intensity profiles of the fluorescence staining along lines crossing the nuclei are shown.