Figure 5
From: Evidence for biphasic uncoating during HIV-1 infection from a novel imaging assay
The kinetics of HIV-1 RNA staining is affected by rhTRIM5α/TRIMCyp but independent of reverse transcription. (A) TZM-bl cells or TZM-bl cells expressing rhTRIM5α were infected with WT HIV-1 and stained for EU at multiple time points. The inset shows staining from a second experiment at earlier time points. (B) OMK cells were infected with WT HIV-1 in the presence or absence of CsA and stained for EU at multiple time points. (C) TZM-bl cells were infected with WT HIV-1 or the D443N RT mutant and stained for EU at multiple time points. 0.6 - 13.5 vRNA puncta were detected per cell. Data represent the mean ± SEM of at least 4 fields and the first time point was normalized to 1. Asterisks denote statistically significant p values (p < 0.05) at each time point between the two lines. The graphs are representative of 2 independent experiments.