Figure 4

Quantification of viral proteins co-localized with WT HIV-1 RNA over time in cells. (A) After HIV-1 infection of TZM-bl cells, RNA staining was counted alone and when co-localized with CA, GFP-Vpr, or NC at 15 min and 60 min post-infection. The percentage of co-localized events for at least four fields was calculated. (B) CA, GFP-Vpr, and NC puncta were quantified at multiple time points post-infection. The first time point was normalized to 1. Data represent the mean ± SEM of at least 4 fields. Asterisks denote statistically significant p values (p < 0.05) between time points for each viral protein or at each time point, comparing NC with CA or with GFP-Vpr. NS denotes a non-significant p value (>0.05). Results are representative of 2 independent experiments.