Figure 3

EU-labeled WT HIV-1 RNA is co-localized with NC in cells. TZM-bl cells were infected for 1 h with EU-labeled virus containing GFP-Vpr (green) and stained for (A) RNA (cyan) and CA (red) or (B) RNA (cyan) and NC (red). DAPI was used to stain nuclei (blue). Cells were then visualized by fluorescent confocal microscopy. Arrows indicate co-localization of vRNA with CA or NC staining when both colors are merged (white). Results are representative of multiple independent experiments.