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Figure 2 | Retrovirology

Figure 2

From: Evidence for biphasic uncoating during HIV-1 infection from a novel imaging assay

Figure 2

EU-labeled HIV-1 can be visualized intracellularly. (A) RNA staining (red) of TZM-bl cells after infection for 2 h with equal multiplicities of infection of EU-labeled virus (labeled during the first 16 h of transfection) or unlabeled virus. (B) Viral membrane-associated S15-mCherry (red) is incorporated into MS2-GFP+ (green) virions prior to infection (top), but is not co-localized with EU-labeled WT HIV-1 RNA staining (green) after incubation with cells (bottom). Viruses labeled with EU and S15-mCherry were used to infect TZM-bl cells for 1 h and stained for EU. DAPI was used to stain nuclei (blue). MS2-GFP or EU and S15-mCherry are shown individually and overlayed together (merge). The panels are representative of multiple fields.

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