Figure 5

Equal elimination of resting and activated CD4⁺T cells. In a subset of our ES cohort, target CD4⁺ T cells analyzed in the suppression assay were also stained with anti-HLA-DR, anti-CD25, and anti-CD69 antibodies and gated using the FACSDiva software to delineate resting and activated CD4⁺ cell populations. The level of elimination of untreated and EFV treated resting (blue diamonds), activated (red squares), and unfractionated (all CD3⁺/CD8- targets; orange circles) target cells was calculated at various effector to target ratios. This analysis was performed at 18 (top panels) and 72 hours (bottom panels) after infection. No statistically significant differences in the levels of elimination between each of the populations were observed.