INI1/hSNF5-interaction defective HIV-1 IN mutants exhibit impaired particle morphology, reverse transcription and integration in vivo

Table 2 Summary of effect of IID-IN mutations on various stages of HIV-1 replication relative to that of the wild type

	% activity of mutants relative to wild type (average of three experiments)^a
Mutation	Binding to INI1	Infection in CEM-GFP (day 10)	Infection in HeLa (24 hr.)	Early RT at 6 hr	Late RT at 6 hr	2LTR circle formation (at 12 hr.)	Integration (at 24 hr.)
IN (WT)	100	100	100	100	100	100	100
H12Y	20.60	0.12	0.07	11.12	7.15	0	0
Q137R	10.00	0.09	0.40	29.89	12.10	30.62	5.42
D202G	32.00	0.09	0.14	14.71	14.74	48.58	1.34
K71R	46.00	3.74	2.52	38.13	27.73	150.78	61.64
S147G	77.00	29.83	6.93	74.01	124.82	164.75	33.29
K111E	ND	29.26	4.68	71.25	104.25	47.07	65.30
D116A	ND	0.11	3.36	79.39	60.15	13369.26	1.44

^a % activity of mutants was determined by considering the activity of wild type as 100%.

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