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Table 2 Summary of effect of IID-IN mutations on various stages of HIV-1 replication relative to that of the wild type

From: INI1/hSNF5-interaction defective HIV-1 IN mutants exhibit impaired particle morphology, reverse transcription and integration in vivo

  % activity of mutants relative to wild type (average of three experiments)a
Mutation Binding to INI1 Infection in CEM-GFP (day 10) Infection in HeLa (24 hr.) Early RT at 6 hr Late RT at 6 hr 2LTR circle formation (at 12 hr.) Integration (at 24 hr.)
IN (WT) 100 100 100 100 100 100 100
H12Y 20.60 0.12 0.07 11.12 7.15 0 0
Q137R 10.00 0.09 0.40 29.89 12.10 30.62 5.42
D202G 32.00 0.09 0.14 14.71 14.74 48.58 1.34
K71R 46.00 3.74 2.52 38.13 27.73 150.78 61.64
S147G 77.00 29.83 6.93 74.01 124.82 164.75 33.29
K111E ND 29.26 4.68 71.25 104.25 47.07 65.30
D116A ND 0.11 3.36 79.39 60.15 13369.26 1.44
  1. a % activity of mutants was determined by considering the activity of wild type as 100%.