| |
% activity of mutants relative to wild type (average of three experiments)a
|
|---|
|
Mutation
|
Binding to INI1
|
Infection in CEM-GFP (day 10)
|
Infection in HeLa (24 hr.)
|
Early RT at 6 hr
|
Late RT at 6 hr
|
2LTR circle formation (at 12 hr.)
|
Integration (at 24 hr.)
|
|---|
|
IN (WT)
|
100
|
100
|
100
|
100
|
100
|
100
|
100
|
|
H12Y
|
20.60
|
0.12
|
0.07
|
11.12
|
7.15
|
0
|
0
|
|
Q137R
|
10.00
|
0.09
|
0.40
|
29.89
|
12.10
|
30.62
|
5.42
|
|
D202G
|
32.00
|
0.09
|
0.14
|
14.71
|
14.74
|
48.58
|
1.34
|
|
K71R
|
46.00
|
3.74
|
2.52
|
38.13
|
27.73
|
150.78
|
61.64
|
|
S147G
|
77.00
|
29.83
|
6.93
|
74.01
|
124.82
|
164.75
|
33.29
|
|
K111E
|
ND
|
29.26
|
4.68
|
71.25
|
104.25
|
47.07
|
65.30
|
|
D116A
|
ND
|
0.11
|
3.36
|
79.39
|
60.15
|
13369.26
|
1.44
|
- a % activity of mutants was determined by considering the activity of wild type as 100%.
