INI1/hSNF5-interaction defective HIV-1 IN mutants exhibit impaired particle morphology, reverse transcription and integration in vivo

Table 1 Surface accessibility (SA) and phylogenetic conservation of IN residues implicated in INI1 binding

	Surface accessibility^a						% identity among lentiviruses^b
HIV-1 IN residue	IN dimer		IN tetramer				HIV-1 and SIV_cpz	Non-HIV-1/SIV_cpzprimate lentiviruses
	A	B	A	B	C	D
K71	118	79	90	94	99	99	98	85
K111	65	81	122	115	138	113	93	32
Q137	71	102	59	56	47	56	91	24
S147	71	N/A	N/A	N/A	N/A	N/A	100	100
D202	71	61	68	50	55	50	100	14

^aDetermined using PISA program based on IN crystal structures of dimer and tetramer (PDB#s 1ex4 and 1k6y, respectively).
^bDetermined using HIV Sequence Compendium (2010).

ISSN: 1742-4690