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Figure 7 | Retrovirology

Figure 7

From: INI1/hSNF5-interaction defective HIV-1 IN mutants exhibit impaired particle morphology, reverse transcription and integration in vivo

Figure 7

Protein-protein interactions of IID-IN mutants with RT and various IN binding host factors. In vitro pull-downs of IID-IN mutants with GST-tagged IN binding partners were carried out using His6-tagged IN (WT or IID-IN mutants) and GST (A), GST- SAP18 (B and C), GST-LEDGF (D and E), GST-GEMIN2 (F and G), and GST-RT (H and I). 5μg of GST-tagged proteins were used for each pull-down reaction. Left side panels (A, B, D, F and H) illustrate Western and Coomassie stained gel to indicate amount of loaded and bound proteins from a representative experiment. Right side panels (C, F, G and I) illustrate graphic representation of relative binding of IID-IN mutants compared to that of WT. Quantitation of binding was carried out by normalizing against total IN loaded for each reaction (C, E, G, I). The graphs (C, E, G and I) represent averages of three independent experiments (Mean +/- SEM).

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