Figure 3

IID-IN mutants are defective for early post entry events. A- C. Effect of IID-IN mutations on early RT. Using genomic DNA from 293T cells infected with HIV-Luc harboring WT-IN, IID-IN, or catalytic mutant D116A, early RT products were quantified by qRT-PCR at indicated times post-infection. Effects of highly defective IID-IN mutants on early RT are shown in (A) and partially defective mutants in (B). C. Relative effect of IID-IN mutations on early RT as compared to WT. Data represents amounts at 6 h post-infection averaged from three independent experiments (Mean +/- SEM). D- F. Effect of IID-IN mutations on late RT. As in panels A-C, late RT products in infected cells were quantified via qRT-PCR at indicated times post-infection. Effects of highly defective IID-IN mutations on late RT are shown in (D) and partially defective mutants in (E). F. Relative effect of IID-IN mutants on late RT as compared to WT. Data represents amounts at 6 h time post-infection averaged from three independent experiments (Mean +/- SEM). G- I. Effect of IID-IN mutations on formation of 2LTR circles. 2LTR products in infected cells were quantified via qRT-PCR at 12 h post-infection. The data represents average of three independent experiments (Mean +/- SEM). Effects of highly defective IID-IN mutations on 2LTR circle formation are shown in (G) and partially defective mutations in (H). I. Relative effect of IID-IN mutations on 2LTR circle formation as compared to WT. Data represents amount of 2LTR products at 12 h post-infection averaged from three independent experiments (Mean +/- SEM). J. The effect of IID-IN mutations on integration. Alu-Gag products were quantified in infected cells via qRT-PCR at 24 hours post-infection. Data are compared to WT and represents average of three independent experiments (Mean +/- SEM).