Enzyme
|
IC50(nM) of EFdA-TP ± SD (Fold change)
|
Hypersusceptibility enhancement in the presence of ATP
|
---|
|
Without ATP
|
With 3.5 mM ATP
| |
---|
WT RT
|
186 ± 40a (1)b
|
318 ± 99a (1)b
|
1c
|
K65R RT
|
125 ± 28a (0.7)b
|
131 ± 28a (0.4)b
|
1.8c
|
- aThe data show “mean value ± standard deviation” obtained from the results of at least four independent experiments (P < 0.011).
- bThe relative increase in IC50 value in K65R RT compared with WT RT without, or with ATP is given in parentheses.
- cThe effect of ATP-based excision on hypersusceptibility is calculated by the relative change in IC50 for K65R compared to WT RT without ATP/the relative change in IC50 for K65R compared to WT RT with ATP.