Figure 5
BST-2/tetherin-mediated restriction on cis -infection from matured infected DC. (A) 1 × 105 DC, previously treated (or not) with IFN-α or LPS, were left uninfected (NI) or infected for 3 days with HIV-WT, HIV-ΔVpu or HIVF522Y viruses in parallel with Vpx-VLPs transduction. Cells were then co-cultured with 1 × 105 autologous CD4+T lymphocytes. HIV-1 transfer on T lymphocytes was scored by FACS analysis of CD3+p24gag+FITC cells, three days post-transfer. (B) Quantification of viral transfer from treated DC as indicated was obtained from 5 different experiments for NT and IFN-α conditions and 3 independent experiments for LPS condition. (C) Percentage of DC-mediated HIV-ΔVpu transfer for each condition was then measured and depicted in the lower graph.