Figure 2
LPS-induced BST-2/tetherin restriction activity in HIV-1 infected DC. (A) 2 × 105 DC, previously treated (or not) with IFN-α or LPS, were left uninfected (NI) or infected for 3 days with HIV-WT, HIV-ΔVpu or HIV-F522Y viruses in parallel with SIV3 (+Vpx) transduction. Infection rates in DC were scored by FACS analysis of BST-2/tetherin+/p24gag+ cells, three days post-infection. (B) TZM-bl cells were incubated for 48 hours with 1/10 volume of supernatant from HIV-WT or HIV-ΔVpu infected DC, previously untreated (NT) or treated with IFN-α or LPS, and harvested three days post-infection. Infectious units (IU/ml) were quantified as above in NT DC and treated DC (from 5 and 3 experiments respectively). (C) Supernatants from HIV-WT or HIV-ΔVpu infected DC, previously untreated (NT) or treated with IFN-α or LPS, were harvested three days post-infection and HIV-p24 levels were quantified by ELISA. (D) Graph represents the percentage of HIV-ΔVpu / HIV-WT infectivity ratios from treated DC as indicated and compared to Hela cells.