Figure 3From: NK cells improve control of friend virus infection in mice persistently infected with murine cytomegalovirusEnhanced numbers of NK cells were determined in persistently mCMV infected compared to naïve mice. IFNγ production by a) CD3-, DX5+, NK1.1+, b) Ly49H+ CD3-, DX5+ NK1.1+ and c) Ly49H- CD3-, DX5+, NK1.1+ cells were determined in spleens of naïve or day 4.5 post FV infection in naïve (white bar) and persistently mCMV (black bar) infected mice. d-f) NK cells were characterized in the spleen of naïve and persistently mCMV infected mice. d) Absolute numbers of NK cells were determined by gating of NK1.1+ DX5+ CD3- cells. Absolute numbers of CD62Llow (e) and MFI of KLRG-1 (f) gated on NK1.1+ DX5+ CD3- cells were analyzed between the different groups. Data are pooled from d) 4–5 independent experiments with 9–16 mice per group, e,f) 3 independent experiments with 8–16 mice per group. Statistical analysis: unpaired t-test. Statistically significant differences between the groups are indicated by * p < 0.05, ** for p < 0.001, *** for p < 0.0001.Back to article page