Figure 4
From: LEDGINs inhibit late stage HIV-1 replication by modulating integrase multimerization in the virions
Reduction of virion replication capacity by LEDGINs is integrase dependent. Detailed analysis of the late effect of LEDGINs on the replication capacity of different HIV-1 strains (NL4.3, LEDGIN-resistant NL4.3A128T or HXB2D): (A) in HeLaP4 cells as determined by measuring beta-galactosidase activity 24 hpi (n = 2), (B) in MT-4 cells by calculating the TCID50/ml 5 dpi (n = 4), and (C) in PBMC by monitoring p24 level in the supernatant (n = 2–4). (D) Effect of CX05045 on HIV-2 and SIVmac251 production and infectivity. The replication capacity of the viruses was determined by scoring CPE in MT-4 cells on day 5 postinfection as determined by relative to TCID50/ml (n = 2). All data represent mean values ± standard deviation. **p < 0.001; 2-way ANOVA.