Figure 1
From: High level of susceptibility to human TRIM5α conferred by HIV-2 capsid sequences
Chimeric viruses carrying HIV-2 CA sequences. The HIV-1 pNL4-3-ΔENV-LucR vector, in which the SacII and XmaI restriction sites were introduced, was used as background to express a variety of HIV-2 and SIV CA sequences. The aminoacid alignment of the extended capsid-coding region of the HIV-1 NL4-3, and the HIV-2 strains GL-AN and ROD10 is shown. The HIV-1 Gag sequences recognized by the HIV-1 protease are highlighted in grey, and the MA/CA and CA/p2 cleavage sites are indicated. The arrows underline the sequences of primers carrying the SacII and XmaI restriction sites, which were used to clone the HIV-2 CA sequences. The aminoacids present into wild-type or chimeric pNL4-3-ΔENV-LucR are in bold. MA, matrix; CA, capsid.