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Figure 3 | Retrovirology

Figure 3

From: Platelet activation suppresses HIV-1 infection of T cells

Figure 3

Supernatants from activated platelets inhibit HIV-1 but not HIV-2 and SIV entry, independent of viral strain and coreceptor tropism. (A) Supernatants from activated platelets efficiently inhibit cellular entry of HIV-1 but not HIV-2 and SIV. TZM-bl indicator cells were preincubated with the supernatants from TRAP-activated platelets (A-PLT Sup) or TRAP containing PBS (TRAP) or PBS. Subsequently, the cells were infected with pseudotypes bearing the indicated Env proteins or with replication competent HIV-2 Rod and SIVmac 239 and β-galactosidase activity in cell lysates was measured. The results ± SD of a representative experiment performed in triplicates are shown. Similar results were obtained in a separate experiment. (B) Inhibition of HIV-1 entry by supernatants from activated platelets is independent of the viral coreceptor tropism. The experiment was performed as described for (A) but HIV-1 NL4-3 chimeras harboring the V3 loops of the indicated primary HIV-1 isolates were used for infection of TZM-bl cells and luciferase activities in cell lysates were quantified. The results of a single representative experiment performed in triplicates are shown; error bars indicate SD. Similar results were obtained in a separate experiment. (C) Different molecular clones of HIV-1 are inhibited by the supernatants from activated platelets. The experiment was performed as in (B), but the indicated HIV-1 infectious clones were used. The average of two to four independent experiments performed in triplicates is shown, error bars indicate SEM.

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