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Figure 5 | Retrovirology

Figure 5

From: Forced virus evolution reveals functional crosstalk between the disulfide bonded region and membrane proximal ectodomain region of HIV-1 gp41

Figure 5

Biochemical analysis of selected revertant clones. (A) Western blotting. At 48-h posttransfection, pΔKADenv–transfected 293T cells were lysed and subjected to reducing SDS-PAGE followed by western blotting with DV-012 to gp120 (upper panel) and mAb C8 to gp41 (lower panel). (B) gp120-gp41 association was determined as for Figure 1B. gp120-shedding index was calculated according to the formula: ([mutant gp120]supernatant x [WT gp120]cell)/([mutant gp120]cell x [WT gp120]supernatant) [12]. (C) Characterization of virions produced by pAD8 infectious clones. Pelleted HIV-1 virions were analysed by Western blotting using DV-012 (upper panel) and pooled IgG from HIV-1-infected persons (lower panel). gp120 and p24 band intensities were determined using a Licor Odyssey scanner.

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