Figure 3

Replication of representative WLKD-1 (A) and WLKD-2 (B) clones in U87.CD4.CCR5 cells. Viruses produced in 293T cells were normalized according to RT activity and used to infect U87.CD4.CCR5 cells. Reverse transcriptase activity in culture supernatants was measured at days 3, 7, 10 and 14. The mean RT activity ± standard deviation of triplicate samples is shown. (C) Single-cycle infectivity was determined in U87.CD4.CCR5 cells infected with Env-pseudotyped luciferase reporter viruses at 48-h postinfection. Luciferase activity was normalized against the RT activity present in each virus inoculum. The mean RLU ± standard errors of 3 independent assays are presented here. **, P < 0.01, unpaired t test assuming unequal variances. (D) Serial 10-fold dilutions of Env-pseudotyped luciferase reporter viruses were added to U87.CD4.CCR5 target cells and luciferase activity determined 48 h later. The mean RLU ± standard deviations from a representative experiment are presented.