Figure 7
Particle stiffness regulates immature entry mediated by VSVg. Varying amounts of GFP-TM1 were incorporated into immature or mature HIV-1 bearing VSVg. Anti-VSVg antibody was used to quantify VSVg levels by WB. Viral entry activity was normalized by VSVg concentration for each virus to obtain its specific entry activity. Error bars indicate the SEM. A) Virion stiffness or B) specific viral entry activity is plotted against GFP-TM1 incorporation levels normalized to corresponding immature/mature virus with 100% GFP-TM1 plasmid input. Immature (orange open diamonds) and mature (blue open circles) HIV-1 bearing VSVg and GFP-TM1 are shown. Specific entry activity is normalized to the corresponding immature or mature virus with no GFP-TM1. At least 16 particles were measured for each stiffness value.