Figure 3

ΔCT coexpression slightly increases GFP-TM1 incorporation. GFP-TM1 was incorporated in immature HIV-1 with or without ΔCT Env. 1X corresponds to 100% GFP-TM1 plasmid input amount. For 0.5X or 1X GFP-TM1, ΔCT plasmid input was replaced by the same amount of an inert bacterial vector (pCDF-BS) to balance plasmid mass during transfection. Input amount of all other plasmids (i.e., genome and GFP-TM1) remain the same with the corresponding viruses bearing ΔCT. CT incorporation levels are normalized to that of immature WT. Error bars indicate the SEM.