Figure 10

Optimization of BlaM viral entry assay. A). Immature ΔCT HIV-1 viruses. BlaM signal is plotted against relative amount of loaded virus. B). Immature HIV-1 bearing titrating amounts of ΔCT Env. Entry signal per virion is plotted against gp120 per virion (determined by WB), both normalized to virus with 100% ΔCT plasmid input. BlaM assay signal increases proportionally to gp120 concentration (linear fit). C). Immature HIV-1 bearing titrating amounts of VSVg. Entry signal per virion is plotted against VSVg per virion, both normalized to virus with 100% VSVg plasmid input. BlaM assay signal increases proportionally to VSVg concentration (linear fit). D). Immature HIV-1 bearing different levels of BlaM. Entry signal per virion is plotted against BlaM per virion, both normalized to virus with 100% BlaM plasmid input. 2–fold variation of BlaM concentration minimally changes the entry assay signal. Error bars indicate the standard error of the mean (SEM).