Figure 4
From: Gelsolin activity controls efficient early HIV-1 infection
Gelsolin functional overexpression impairs HIV-1 Env-gp120-mediated CD4-CXCR4 and CD4-CCR5 redistribution in permissive lymphocytes. (A) Left, HIV-1 Env-gp120-induced redistribution of CD4-CXCR4 to the cell pole in CEM.NKR-CCR5 cells expressing gelsolin-EGFP or EGFP, 1 hour of after exposure to rs-gp120IIIB (5 μg/mL) at 37°C. A series of xy midsections and merged images for CD4-CXCR4/gelsolin-EGFP co-localization at cell-surface are shown. Scale bar = 5 μm. One representative experiment of three is shown. Right, HIV-1 Env-gp120-induced CD4-CXCR4 capping is reflected as the percentage of 200 cells counted for each experimental condition, where the data represent the results of three independent experiments. (B) Analysis and quantification of HIV-1 Env-gp120-induced CD4-CCR5 redistribution in permissive cells expressing gelsolin-EGFP or EGFP. A series of xy midsections and merged images show the co-localization of CD4-CCR5/gelsolin-EGFP at the cell surface. Scale bar = 5 μm. One representative experiment of three is shown. Right, HIV-1 Env-gp120-induced CD4-CCR5 capping is shown as the percentage of 200 cells counted for each experimental condition, where the data represent the results of three independent experiments.