Figure 3
From: Gelsolin activity controls efficient early HIV-1 infection
Effect of gelsolin overexpression on HIV-1 Env-gp120-induced cortical F-actin redistribution in permissive lymphocytes. (A) Confocal analysis of HIV-1 Env-gp120-induced F-actin redistribution in CEM.NKR-CCR5 cells expressing free EGFP (controls) or gelsolin-EGFP. In control cells, rs-gp120IIIB (5 μg/mL) triggered F-actin capping (see white asterisk). F-actin expression was clearly visible in control conditions (see white arrowhead in pEGFP-N1 images) but was reduced in cells expressing gelsolin-EGFP. Merged images show the cortical co-localization of F-actin and gelsolin-EGFP or free EGFP. Right histograms, rs-gp120IIIB-triggered cortical F-actin capping is represented as the percentage of 200 cells counted for each experimental condition. Scale bar = 5 μm. A series of xy midsections from one representative experiment out of three is shown. (B) Similar data was obtained by confocal analysis of HIV-1 Env-gp120-induced F-actin redistribution in CEM.NKR-CCR5 cells expressing free EGFP (controls) or co-expressing both free gelsolin and EGFP. In control cells, rs-gp120IIIB (5 μg/mL) triggered F-actin capping (see white asterisk). F-actin expression was clearly visible in control conditions (see white arrowhead in EGFP-pSUPER images) but was reduced in cells expressing EGFP-pSUPER-gelsolin. Merged images show the cortical co-localization of F-actin and gelsolin or free EGFP. Cortical F-actin capping (only observed in EGFP-pSUPER condition) or distribution is represented as the percentage of 200 cells counted for each experimental condition. Scale bar = 5 μm. A series of xy midsections from one representative experiment out of three is shown.