Dynamic electrophoretic fingerprinting of the HIV-1 envelope glycoprotein

Retrovirology

Table 1 Electrophoretic mobility (EM) of HIV and Influenza envelope protein constructs

Electrophoretic mobility of viral envelope proteins at two salinities (μmcm/Vs)
pH	BX08 gp120		BX08 gp140		CN54 gp140		H3N2 HA
pH	2.75	4.15	2.75	4.15	2.75	4.15	2.75	4.15
4.5	−0.21	−0.44	0.23	0.00	−0.22	−0.60	ND	−1.47
5.5	−1.09	−0.41	−0.47	−0.27	−0.63	−0.81	ND	−1.56
6.5	−1.40	−0.80	0.53	0.13	−0.40	−0.60	ND	−1.04
7.5	−2.34	−0.94	0.36	0.69	0.44	−0.63	ND	1.44
8.5	−2.27	−1.15	−0.01	0.17	−0.88	−0.79	ND	0.26

EM measurements were made for each protein at a range of pH and pλ conditions; a subset of the observed mobilities is shown here. ND = not determined.

ISSN: 1742-4690