Figure 4

dNTP levels in CD4+ activated T cells are unaffected by IFN-α. (A) CD4+ activated T cells derived from 4 donors’ PBMC were either mock treated, or treated with a 1000 U/ml dose of IFN-α for 24 h. The cells were then challenged with a HIV-1-GFP reporter virus at MOI 4. The percentage of infection (% GFP + cells) was measured 3 days post-transduction by flow cytometry. (B) The intracellular dATP and dTTP levels were measured for one donor using the single nucleotide-incorporation assay.