The NTD-CTD intersubunit interface plays a critical role in assembly and stabilization of the HIV-1 capsid

Retrovirology

Table 1 Summary of viral mutant phenotypes

Virus	Mutation Location	Single-cycle infectivity (% of WT ± SD)	Replication in CEM cells^@	% of mature cores^#	Presence of cones/tubes	% core-associated CA (±SD)	Disassembly *in vitro*	Abro-gation of restriction	Reverse transcription in cells (% of WT ± SD)
WT	-	100	+++	50	+	14 ± 2	Normal	Yes	100
H62A	Helix 4	1.0 ± 0.3	-	10	-	1.1 ± 0.4	ND	No	1.3 ± 0.5
Q63A	Helix 4	27 ± 3	+	15	+	5.2 ± 0.2	Accelerated	Yes	50 ± 14
A64D	Helix 4	0 ± 0.1	-	35	-	1 ± 0.6	ND	No	1.3 ± 0.5
M68A	Helix 4	1.0 ± 1	-	10	-	0.8 ± 0.3	ND	No	1.3 ± 0.5
E71A	Helix 4	18 ± 4	-	20	+	6.3 ± 2	Normal	Yes	49 ± 6.2
E75A	Helix 4	13 ± 3	+	20	+	3.6 ± 1	Accelerated	Yes	34 ± 6.2
K140A	Helix 7	1.0 ± 0	-	20	-	0 ± 0	ND	No	1.0 ± 0.8
R162A	Helix 8	0 ± 0.2	-	35	+	0.2 ± 0.1	ND	No	1.7 ± 0.5
V165A	Helix 8	1.0 ± 1	-	25	a	31 ± 4	Slowed	Yes	2.0 ± 0.8
D166A	Helix 8	1.0 ± 1	-	35	-	1 ± 0.1	ND	No	1.3 ± 0.5
R167A	Helix 8	11 ± 2	+	35	+	6.4 ± 1.1	Accelerated	No	32 ± 5.2
Q176A	Helix 8	25 ± 3	++	30	+	8.1 ± 2	Normal	Yes	83 ± 13
Q179A	Helix 8	62 ± 12	+++	35	+	13 ± 2	Normal	Yes	94 ± 7.9
E180A	Helix 8	4 ± 1	-	20	-	5.1 ± 1.4	Normal	Yes	30 ± 1.7
M215A	Helix 11	0 ± 0.3	-	20	-	0.5 ± 0.1	ND	No	1.5 ± 0.5

^@ +++, replicates like wild type; ++, 3 days delayed; +, 15–18 days delayed; -, no replication within 30 days.
^# mature cores exhibit an electron-dense core region, which is either bar-shaped, conical or spherical.
ND; not determined.
a: conical but morphologically aberrant cores.

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