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Figure 5 | Retrovirology

Figure 5

From: Restriction of diverse retroviruses by SAMHD1

Figure 5

Vpx does not enhance PFV infection of MDM. (A) MDM were preincubated with Vpx-containing (+Vpx) or control VLP (−Vpx) or medium and were not infected (mock) or infected with PFV-GFP or HIV-GFP in triplicate at MOI = 1. Three days postinfection, the infected cells were quantified by flow cytometry. One out of three independent experiments is shown. (B) MDM were incubated with Vpx-containing or control VLP or medium (PFV) and then not infected (mock) or infected with PFV reporter virus at an MOI =1. After 24 and 48 h, cellular DNA was isolated and PFV DNA was quantified by qPCR. AZT was added to one well treated with Vpx-containing VLP prior to infection to control for plasmid contamination (AZT). The data are the average of triplicates with error bars indicating the standard deviation. Similar results were obtained with cells from two different donors.

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