Figure 1

SAMHD1 restricts lentiviral infection in myeloid cells. (A) Vpx enhances lentiviral infection in MDM. MDM were preincubated for 2 h with Vpx-containing (+Vpx) or control VLP (−Vpx) and either not infected (mock) or infected with VSV-G-pseudotyped HIV-GFP, FIV-GFP, or EIAV-RFP reporter virus. Reporter virus infection was quantified three days later by flow cytometry. The percentage of infected cells is shown as average of duplicate infections. (B) FACS plot of one donor MDM infected as described in (A). (C) MDDC were incubated with Vpx-containing VLP, control VLP or no VLP for 24. Cell lysates were prepared and analyzed on an immunoblot probed with anti-SAMHD1 MAb or anti-GAPDH MAb. The results shown are representative of results obtained in three independent experiments.