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Figure 8 | Retrovirology

Figure 8

From: The V86M mutation in HIV-1 capsid confers resistance to TRIM5α by abrogation of cyclophilin A-dependent restriction and enhancement of viral nuclear import

Figure 8

Restriction of a spreading CA-V86M HIV-1 infection. Sup-T1 cells transduced with a control shRNA against luciferase (A) or with an shRNA targeting CypA (B) and expressing the indicated TRIM5α proteins were infected once with an identical dose of WT or V86M HIV-1NL4-3 normalized by RT activity. Unbound virus was eliminated 16 h later and infection was allowed to proceed for 45 days. Reverse transcriptase activity in the supernatant of infected cells and of uninfected cells as a control were determined periodically. Background noise as determined from the uninfected cells was subtracted from the other samples at each time-point.

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