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Figure 7 | Retrovirology

Figure 7

From: The V86M mutation in HIV-1 capsid confers resistance to TRIM5α by abrogation of cyclophilin A-dependent restriction and enhancement of viral nuclear import

Figure 7

V86M enhances nuclear transport of HIV-1 DNA in restrictive conditions. TE671 cells expressing the indicated TRIM5α were challenged with WT or V86M HIV-1NL-GFP. An RT inhibitor (nevirapine) control was included to show the absence of contaminating DNA. The levels of HIV-1 late reverse transcription products and 2-LTR circles were determined by real-time quantitative PCR using primers to detect GFP or 2-LTR junctions, respectively. DNA amounts were calculated using serially diluted, linearized DNAs as standards. Data are presented as mean ratio of late RT or 2-LTR DNA copies over the number of GAPDH DNA copies, normalized to the permissive control TRIM5αhu. Shown are values with standard deviations of 3 or 5 independent infections (late RT products and 2-LTR, respectively). Student’s t-test was used to test for significance of data, specifically WT vs V86M viruses in cells expressing R332G-R335G or TRIM5α (p = 0.0020 and p = 0.0015, respectively).

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