Figure 5
V86M HIV-1 restriction by R332G-R335G TRIM5α hu in cat cells is not counteracted by CsA treatment. CRFK cells were transduced with R332G-R335G TRIM5αhu or with the empty vector as control and subsequently challenged with a single dose of WT or V86M HIV-1NL-GFP in presence or absence of 2 μM CsA. Infections were done at a multiplicity of infection of ~0.2 as determined in parental cells infected with the WT virus, and the amounts of viruses used were normalized by RT assay. The percentages of infected cells were determined two days later by FACS.