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Figure 2 | Retrovirology

Figure 2

From: The V86M mutation in HIV-1 capsid confers resistance to TRIM5α by abrogation of cyclophilin A-dependent restriction and enhancement of viral nuclear import

Figure 2

Restriction of CA-V86M HIV-1 by R332G-R335G TRIM5α hu is independent of cyclophilin A. TE671 cells (A) and Sup-T1 cells (B) transduced with either WT TRIM5αhu, R332G-R335G TRIM5αhu or with the “empty” vector were also transduced with shRNAs targeting CypA or the non-relevant luciferase mRNAs. Following antibiotic selection, cells were challenged with a single dose of WT or V86M HIV-1NL-GFP, in the presence or absence of 2 μM CsA. A multiplicity of infection of ~0.2 was used, as determined for the WT virus in parental cells and in the absence of drug. WT and V86M virus preparations were adjusted by RT assay. Cells were analyzed by FACS two days after infection, and the bars show the average % infected cells from 3 infections with standard deviations. Note the difference in scale between the y-axis of different graphs.

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Retrovirology

ISSN: 1742-4690

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