Skip to main content
Figure 1 | Retrovirology

Figure 1

From: The V86M mutation in HIV-1 capsid confers resistance to TRIM5α by abrogation of cyclophilin A-dependent restriction and enhancement of viral nuclear import

Figure 1

V86M mutation in HIV-1 capsid confers partial resistance against R332G-R335G TRIM5α hu . TE671 (A), Sup-T1 (B) and CRFK (C) cells were transduced with either WT TRIM5αhu, R332G-R335G TRIM5αhu, or with the “empty” vector as a control. Following antibiotic selection, cells were infected with multiple doses (A, B) or with a single dose (C) of WT or V86M HIV-1NL-GFP. The two viral preparations had similar virion content as determined by RT assay. The percentage of infected (GFP-positive) cells was determined two days later by flow cytometry. In (C), infections were done at a multiplicity of infection of ~0.1 as determined for the WT virus in parental cells. Bars show the average data from 3 infections with standard deviations (***, P-value < 0.0001).

Back to article page