Figure 4From: Evidence for IFNα-induced, SAMHD1-independent inhibitors of early HIV-1 infectionSAMHD1 is induced by IFNα in U87-MG cells, but Vpx triggered degradation does not rescue IFNα-mediated HIV-1 suppression. A. U87-MG cells were treated for 24 h with IFNα, or not, and incubated with no VLPs, Vpx-VLPs, or control VLPs (Ctrl-VLPs) and then infected with increasing amounts of GFP reporter virus (MOI 0.008 to 1), and GFP expression monitored as before. The mean values from 3 independent experiments are shown. B. Western blot analysis of a parallel samples from A. Protein levels of SAMHD1 were determined and tubulin served as a loading control.Back to article page