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Figure 8 | Retrovirology

Figure 8

From: TNPO3 protects HIV-1 replication from CPSF6-mediated capsid stabilization in the host cell cytoplasm

Figure 8

CPSF6 stabilizes the HIV-1 CA core. (A) Env- HIV-1, pseudotyped with VSV G, and bearing either WT or A105T mutant CA, was incubated with TZM-bl cells stably transduced with CPSF6-358 (+) or empty vector (−), for 4, 10 and 16 hours. As a control, virions lacking VSV G (No VSVG) were also incubated with CPSF6-358 expressing cells. The target cells were lysed and the cytoplasmic fraction (Input) was pelleted through a 50% sucrose cushion to separate the particulate (Pellet) and soluble fractions (Supernatant). The fractions were then analyzed by western blot with an antibody anti-p24. (B) WT or A105T CA mutant viruses, and no VSV G virus, were incubated with control (Ctrl) or TNPO3 KD cells for 12 hours. Separation and analysis of the three fractions was described in (A). (C) WT or A105T CA mutant viruses and Env- virus were incubated with TZM-bl cells stably transduced with rhTRIM5α expressing vector or with an empty vector for 12 hours. Separation and analysis of the three fractions was described in (A). All experiments are representative of at least 2 repetitions.

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