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Table 6 Point errors at drug resistance mutation sites

From: Analysis of 454 sequencing error rate, error sources, and artifact recombination for detection of Low-frequency drug resistance mutations in HIV-1 DNA

Position

Mutation

Run1

Run2 b

Highest of 2 run (95 CI)

21

M41L(A->C)

0.02%

0.01%

0.02% (0.01 to 0.03)

94

K65R(A->G) a

0.31%

0.09%

0.31(0.19 to 0.42)

99

D67W(G->A)

0.07%

0.02%

0.07% (0.01 to 0.13)

109

K70R(A->G)

0.09%

0.06%

0.09% (0.03 to 0.16)

120

L74V(T->G)

0.02%

0.00%

0.02% (0.00 to 0.01)

198

L90I(T->A)

0.00%

0.00%

0.00% (0.00 to 0.03)

209

K103N(A->C)

0.02%

0.01%

0.02% (0.01 to 0.03)

210

K103N(A->T)

0.02%

0.02%

0.02% (0.01 to 0.03)

  1. a Transition errors are in bold.
  2. b Run1 includes MID1 (100% wt), 2 (100% wt), 5 (10% mutant), 7 (1% mutant); Totally 77,673 sequences analyzed . Run2 includes MID1 (100% wt), 2 (100% wt), 5 (100% mutant), 7 (50% mutant), 9 (50% mutant); Totally 313,523 sequences analyzed.