Table 3 Distribution of errors per sequence
Sample (total sequences) | 0 errors | 1 errors | 2 errors | 3 errors | 4 errors | 5 errors | 6–10 errors | >10 errors | All sequences with errors |
|---|---|---|---|---|---|---|---|---|---|
Run1MID1 (8305) | 24.23% | 44.32% | 18.62% | 8.04% | 3.05% | 1.01% | .067% | 0.06% | 6293 (75.77%) |
Run1MID2 (43699) | 25.50% | 14.47% | 36.56% | 15.79% | 4.68% | 1.53% | 1.42% | 0.05% | 32556 (74.50%) |
Run1MID3 (24210) | 67.16% | 24.51% | 5.03% | 1.62% | 0.85% | 0.43% | 0.40% | 0.02% | 7951 (32.84%0 |
Run1MID4 (13466) | 69.69% | 22.70% | 4.60% | 1.45% | 0.63% | 0.40% | 0.52% | 0.00% | 4081 (30.31%) |
Run2MID1 (48825) | 23.23% | 43.26% | 20.37% | 7.09% | 2.59% | 1.27% | 1.80% | 0.40% | 37483 (76.77%) |
Run2MID2 (98785) | 0.12% | 65.26% | 23.88% | 6.17% | 2.33% | 0.96% | 1.08% | 0.20% | 95665 (99.88%) |
Run2MID3 (60477) | 66.18% | 23.57% | 5.90% | 1.96% | 0.79% | 0.51% | 0.98% | 0.09% | 20451 (33.82%) |
Run2MID4 (38970) | 60.18% | 26.66% | 7.64% | 2.42% | 1.03% | 0.70% | 1.27% | 0.12% | 15517 (39.82%) |
Number of sequences (336737) | 113740 | 129224 | 59438 | 19843 | 7031 | 3056 | 3875 | 526 | 222997 (66.22%) |
Percentage in all sequences | 33.78% | 38.38% | 17.65% | 5.89% | 2.09% | 0.91% | 1.15% | 0.16% | Â |