Sample | Fragment | Mixture | Number of sequence | Â | Â | |||
---|---|---|---|---|---|---|---|---|
 |  |  | Total sequence | Wild type | Mutant | Recombinant | Observed rate of recombinants | Corrected rate of recombinants |
Run1 | ||||||||
MID1 | 1 | 100% wild type | 8503 | 8483 | 0 | 20 | 0.24% | 0.00% |
MID2 | 1 | 100% wild type | 43699 | 43480 | 2 | 217 | 0.50% | 0.00% |
MID5 | 1 | 10% mutant | 15923 | 12485 | 2039 | 1399 | 8.79% | 8.42% |
MID7 | 1 | 1% mutant | 9548 | 9329 | 98 | 121 | 1.27% | 0.90% |
MID3 | 2 | 100% mutant | 24210 | 1 | 24127 | 82 | 0.34% | 0.00% |
MID4 | 2 | 100% mutant | 13466 | 0 | 13432 | 34 | 0.25% | 0.00% |
MID10 | 2 | 10% mutant | 49169 | 35477 | 10156 | 3536 | 7.19% | 6.90% |
MID11 | 2 | 1% mutant | 12070 | 11681 | 171 | 218 | 1.81% | 1.51% |
Run2 | ||||||||
MID1 | 1 | 100% wild type | 48828 | 48735 | 0 | 90 | 0.18% | 0.00% |
MID2a | 1 | 100% wild type; Clone DNA/no PCR | 98785 | 98670 | 2 | 113 | 0.11% | 0.00% |
MID5 | 1 | 100% mutant | 52287 | 0 | 51903 | 384 | 0.73% | 0.00% |
MID7 | 1 | 50% mutant | 58882 | 18565 | 31388 | 8929 | 15.16% | 14.82% |
MID9 | 1 | 50/50 mix RNA | 54744 | 14151 | 27648 | 12945 | 23.65% | 23.30% |
MID3 | 2 | 100% mutant | 60477 | 6 | 60297 | 174 | 0.29% | 0.00% |
MID4 | 2 | 100% wild type | 38970 | 38705 | 0 | 265 | 0.68% | 0.00% |
Run 3 | ||||||||
MID11 | 1 | 50% mutant | 62437 | 21163 | 40777 | 497 | 0.78% | 0.43% |
MID12 | 1 | 50% mutant | 122327 | 23589 | 83134 | 15604 | 12.00% | 11.65% |