Figure 4

Point error and indel error estimation. (A). Point error distribution in Run1 fragment 1. The data are from Run1 MID1, 2, 5, 7, 8, and 9. The X axis is the nucleotide position of sequences, with position 1 corresponding to nucleotide 101 in BH10 RT [29]. The Y axis is the mean error rate of all samples at each position (blue diamonds). The error bars correspond to 1 standard deviation. The orange diamonds at the top show the locations of drug resistance sites. The numbers with arrows indicate nucleotide positions of sites with high error rates. The gray boxes show the 20 nucleotide PCR primer regions. (B) Indel error distribution in Run1 fragment 1, combined MID1, 2, 5, 7, 8, and 9. The Y axis is the mean indel error rate of all samples at each position (blue squares). The error bars correspond to 1 standard deviation of the error rate of each position. Other symbols are as in panel A. The numbers in parentheses indicate the number of As in homopolymer regions. (C) Point error distribution in Run2 fragment 1. The data are from Run2 MID1, 5, 7, and 9. Conventions are as in panel A. The pink squares show point errors from cloned sample DNA (MID2). Blue diamonds show the mean error rate of all other samples at each position. (D) Indel error distribution from Run2 MID1, 5, 7, and 9. Conventions as in panel B Pink squares show the indel errors of cloned sample DNA. Blue squares are the mean indel error rate of all other samples at each position. The colored symbols and vertical lines after the second gray box show the average errors and standard deviations.