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Figure 7 | Retrovirology

Figure 7

From: Transcriptional profiling reveals molecular signatures associated with HIV permissiveness in Th1Th17 cells and identifies Peroxisome Proliferator-Activated Receptor Gammaas an intrinsic negative regulator of viral replication

Figure 7

PPARγ pathway negatively regulates HIV replication in T-cells at levels post-entry and prior integration. (A) CD4+ T-cells were isolated, activated via TCR and nucleofected with PPARγ vs. NT1 siRNA as described in Figure 6. Cells were exposed to single round HIV-VSVG-GFP at 24 h post-nucleofection and cultured for three days in the presence of IL-2. (B) Shown are levels of integrated HIV-DNA (n = 5). (C) Shown is the frequency and MFI of GFP-expressing cells in one representative subject (upper panels) and statistical analysis in n = 5 different subjects (lower panels). Paired t-Test values are indicated on the graphs.

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