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Figure 4 | Retrovirology

Figure 4

From: Transcriptional profiling reveals molecular signatures associated with HIV permissiveness in Th1Th17 cells and identifies Peroxisome Proliferator-Activated Receptor Gammaas an intrinsic negative regulator of viral replication

Figure 4

RT-PCR validation of differentially expressed genes. Total RNA was extracted from CD3/CD28-stimulated matched Th1Th17 and Th1 subsets. The expression of CXCR6, PPARγ, ARNTL, PTPN13, MAP3K4, CTSH, SERPINB6, PTK2, and ISG20 mRNA was quantified by SYBR green real time RT-PCR. Quantification was performed relative to a standard curve generated based on cDNA specific for each transcript. The expression of each gene was normalized to the 28S rRNA internal control and expressed as fgs RNA of a target gene per 1 ng 28S rRNA. Depicted are results obtained with matched Th1Th17 vs. Th1 cells from five different HIV-uninfected individuals. Paired t-Test values are indicated on the graphs.

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Retrovirology

ISSN: 1742-4690

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